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Case Studies

Spectroscopy Mapping of Single Fluorescent Microspheres (Chen)

Spectroscopy Mapping of Single Fluorescent Microspheres

Researcher Objectives

Associate Professor Lingling Chen at Shenzhen University (Guangdong Province, China) specializes in biomedical imaging and photonics.

“It is very impressive for such a compact system [FERGIE] to achieve diffraction-limited images and spectra. It means we can attain higher spatial resolution, which is very helpful for us to differentiate normal and diseased tissues in future applications. We may use IntelliCal intensity calibration to make the spectra even more accurate.”

 

“FERGIE体积很小,却能实现衍射极限成像和光谱采集,让人印象深刻。这意味着我们在将来的应用中可以更精细地在空间上分辨不同位置的生物组织发出的光谱;我们可能后续会需要用IntelliCal强度校准工具来使得光谱更准确

 

— Associate Professor Lingling Chen

副教授陈玲玲

Recently, Associate Professor Chen performed an experiment to validate mapping capacity using a FERGIE system to take fluorescent spectra of a microsphere sample (see Figure 1).

Click image below to enlarge and see caption.

“For fluorescent spectroscopy mapping applications, acquisition speed is crucial. FERGIE’s frame-transfer readout ensures fast and consecutive capture of many spectra.”

 

“对于荧光光谱面分析应用而言,采集速度是很关键的;FERGIE的帧转移读出方式使得我们可以快速、连续地采集大量光谱”

 

— Associate Professor Lingling Chen
副教授陈玲玲

Click images below to enlarge and see captions.

FERGIE in Action

Associate Professor Chen’s experiment (see Figure 2) utilized FluoSpheres carboxylate-modified microspheres from ThermoFisher Scientific with a 2.0 μm diameter (see Figure 3). The excitation wavelength was 488 nm and the peak emission wavelength was 515 nm. A 10x objective was employed.

The resultant experimental data is presented in Figures 4–6.

“FERGIE works so conveniently with microscopes that the system can be set up in 5 minutes. Its slit design makes switching between the acquisition of images and spectra much easier.”

 

“FERGIE和显微镜连用非常方便,五分钟以内即可搭建完成,另外狭缝的设计使得成像和光谱采集的切换非常简便”

— Guiye Li, Graduate Student
研究生李贵叶

Dr. Lingling ChenAssociate Professor Lingling Chen
College of Optoelectronic Engineering
Shenzhen University, P.R. China

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